Novel Method for Detection of β-Lactamases by Using a Chromogenic Cephalosporin Substrate

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Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.

A new cephalosporin with a highly reactive beta-lactam ring was found to give an immediate color change in the presence of beta-lactamases from many bacteria, including staphylococci, Bacillus species, Enterobacteriaceae, and Pseudomonas. The reaction is confined to organisms producing beta-lactamases, but it is sufficiently sensitive to indicate the presence of this enzyme is small amounts in ...

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CENTA as a Chromogenic Substrate for Studying β-Lactamases

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Susceptible Chromogenic Cephalosporin Reagent

CENTA is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (K maximum ca. 340 nm) to chrome yellow (A maximum ca. 405 nm) concomitant with hydrolysis of the betalactam ring. This compound offers promise as a diagnostic reagent comparable to other chromogens (PADAC and nitrocefin) for the early detection of betalactamase-producing...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1972

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.1.4.283